Hotspot

ycf1

SSC · 121078-126237 · 5160 bp

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Pi 0.00708

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5160
variable_sites: 114
pi: 0.00708
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAGTGGGGAATGGGCATTT	TGAAATGGGTTGCGTTGGTA	45.0	45.0	58.0	58.0	1458-1467 bp	1.0	82.1
ycf1_p2	GCTTTTCTTGACCGCATTCC	TGAAATGGGTTGCGTTGGTA	50.0	45.0	58.0	58.0	1264-1273 bp	1.0	81.9
ycf1_p3	AATTTCCTCGATTCCCACGG	TGAAATGGGTTGCGTTGGTA	50.0	45.0	58.0	58.0	1154-1163 bp	1.0	81.7
ycf1_p4	AGGGACCGTTTAAGAAGGGA	TGAAATGGGTTGCGTTGGTA	50.0	45.0	58.0	58.0	972 bp	0.909091	77.3
ycf1_p5	AAAAGTGGGGAATGGGCATT	TGAAATGGGTTGCGTTGGTA	45.0	45.0	58.0	58.0	1468 bp	0.727273	69.7