Hotspot

psbC

LSC · 33598-35019 · 1422 bp

Back to Aeonium

Pi 0.00434

MarkerSeek score 57.50

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 31
pi: 0.00434
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GAAGGTATTCGTGCGTGGAT	AGCCGAGCCAGAAAAAGAAA	50.0	45.0	58.1	58.0	1428-1563 bp	1.0	85.0
psbC_p2	GTGCAGCTGAAGATCCTGAA	AGCCGAGCCAGAAAAAGAAA	50.0	45.0	57.9	58.0	1484-1619 bp	1.0	84.6
psbC_p3	AAATCCGTGCAGCTGAAGAT	AGCCGAGCCAGAAAAAGAAA	45.0	45.0	57.9	58.0	1490-1625 bp	1.0	84.5
psbC_p4	GAAGGTATTCGTGCGTGGAT	ATGACCCGATCCCACTATGT	50.0	50.0	58.1	57.9	1394-1529 bp	1.0	84.4
psbC_p5	TTCGTTTCCCAGGAAATCCG	AGCCGAGCCAGAAAAAGAAA	50.0	45.0	58.2	58.0	1503-1638 bp	1.0	84.0