Hotspot

ndhA

SSC · 117211-119305 · 2095 bp

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Pi 0.00371

MarkerSeek score 54.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2095
variable_sites: 28
pi: 0.00371
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCCGGTTACCATAGGGAA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.0	56.5	2205-2213 bp	1.0	69.1
ndhA_p2	AACCCGGTTACCATAGGGAA	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.0	57.2	2205-2213 bp	1.0	67.1
ndhA_p3	CCGTACCGTTTGTTGACCAT	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.5	56.5	2233-2241 bp	1.0	65.5
ndhA_p4	AACCCGGTTACCATAGGGAA	ATGGGAGAAGTTGATCGTTGA	50.0	42.9	58.0	57.0	2206-2214 bp	1.0	65.4
ndhA_p5	GAACCCGGTTACCATAGGGA	TGGGAGAAGTTGATCGTTGA	55.0	45.0	58.5	56.5	2206-2214 bp	1.0	65.4