Hotspot

rpl32-trnL-UAG

SSC · 111355-111835 · 481 bp

Back to Aeonium

Pi 0.0106

MarkerSeek score 49.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 481
variable_sites: 13
pi: 0.0106
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGGTAGCG	TTCGAACCGAGATGCTCAAG	55.0	50.0	57.5	58.0	642-664 bp	1.0	78.4
rpl32-trnL-UAG_p2	GGAAGGGATATTGGGTAGCG	TTAGAAGAGAATGTGCCGCC	55.0	50.0	57.5	58.0	669-691 bp	1.0	78.1
rpl32-trnL-UAG_p3	TGGGTAGCGTTAAAAGCCTT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.4	58.0	631-653 bp	1.0	77.6
rpl32-trnL-UAG_p4	TTGGGTAGCGTTAAAAGCCT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.4	58.0	632-654 bp	1.0	77.6
rpl32-trnL-UAG_p5	GGAAGGGATATTGGGTAGCG	AAGCACTGCTTCCTAAGAGC	55.0	50.0	57.5	57.9	625-647 bp	1.0	77.6