Hotspot

petL-petG

LSC · 64377-64550 · 174 bp

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Pi 0.0138

MarkerSeek score 44.30

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 6
pi: 0.0138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ATTGGGCTGAGCAAGATACG	GTCCAACTGATCACCACGTT	50.0	50.0	58.0	58.1	312-314 bp	1.0	80.5
petL-petG_p2	ATTGGGCTGAGCAAGATACG	AAGTCCAACTGATCACCACG	50.0	50.0	58.0	57.8	314-316 bp	1.0	80.1
petL-petG_p3	GCCTTTTTATTGGGCTGAGC	GTCCAACTGATCACCACGTT	50.0	50.0	57.7	58.1	320-322 bp	1.0	78.4
petL-petG_p4	GGCTGAGCAAGATACGACTT	GTCCAACTGATCACCACGTT	50.0	50.0	57.7	58.1	308-310 bp	1.0	78.4
petL-petG_p5	GCCTTTTTATTGGGCTGAGC	AAGTCCAACTGATCACCACG	50.0	50.0	57.7	57.8	322-324 bp	1.0	78.0