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psaA-ycf3

LSC · 41824-42472 · 649 bp

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Pi 0.00723

MarkerSeek score 62.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 18
pi: 0.00723
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	812-841 bp	1.0	82.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	827-856 bp	1.0	82.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	741-770 bp	1.0	82.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	814-843 bp	1.0	81.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TAGCACTTACTCCCGGGAAT	50.0	50.0	57.9	57.8	782-811 bp	1.0	81.7