Hotspot

trnC-GCA-petN

LSC · 27700-28494 · 795 bp

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Pi 0.00663

MarkerSeek score 56.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 16
pi: 0.00663
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTTTCTCTCATTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.0	58.0	893-922 bp	1.0	83.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	837-866 bp	1.0	83.6
trnC-GCA-petN_p3	TTCTCTCATTTTGGCGGCAT	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.2	58.0	891-920 bp	1.0	82.7
trnC-GCA-petN_p4	GTTTCTCTCATTTTGGCGGC	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.0	57.8	913-916 bp	0.909091	76.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	857-860 bp	0.909091	75.9