Hotspot

rpoB-trnC-GCA

LSC · 26528-27620 · 1093 bp

Back to Aeonium

Pi 0.00559

MarkerSeek score 65.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1093
variable_sites: 19
pi: 0.00559
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACCTTCCCTCATTTCC	ATCGGGCGAACATAATACGG	55.0	50.0	57.9	57.9	1143-1252 bp	1.0	83.6
rpoB-trnC-GCA_p2	GTGGACCTTCCCTCATTTCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1092-1201 bp	1.0	83.6
rpoB-trnC-GCA_p3	AGTTCTTCCGTCAAGCCATG	ATCGGGCGAACATAATACGG	50.0	50.0	58.2	57.9	1215-1324 bp	1.0	83.1
rpoB-trnC-GCA_p4	AGTTCTTCCGTCAAGCCATG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1164-1273 bp	1.0	83.1
rpoB-trnC-GCA_p5	GTTCTTCCGTCAAGCCATGA	ATCGGGCGAACATAATACGG	50.0	50.0	58.2	57.9	1214-1323 bp	1.0	83.0