Hotspot

trnK-UUU-rps16

LSC · 4166-4804 · 639 bp

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Pi 0.0037

MarkerSeek score 57.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 6
pi: 0.0037
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTCGTCCAAACTCTACCGAC	TTGAAAAGGGGGCTAAACCG	55.0	50.0	58.0	58.4	835-855 bp	1.0	80.8
trnK-UUU-rps16_p2	CGAATCCCTTGCTTCATCCA	TTGAAAAGGGGGCTAAACCG	50.0	50.0	58.0	58.4	807-827 bp	1.0	80.8
trnK-UUU-rps16_p3	ACGAATCCCTTGCTTCATCC	TTGAAAAGGGGGCTAAACCG	50.0	50.0	58.0	58.4	808-828 bp	1.0	80.8
trnK-UUU-rps16_p4	CGGTACAGACGAATCCCTTG	TTGAAAAGGGGGCTAAACCG	55.0	50.0	58.1	58.4	816-836 bp	1.0	80.5
trnK-UUU-rps16_p5	CAGACGAATCCCTTGCTTCA	TTGAAAAGGGGGCTAAACCG	50.0	50.0	57.9	58.4	811-831 bp	1.0	80.4