Hotspot

psaC-ndhE

SSC · 108815-109281 · 467 bp

Back to Aeluropus

Pi 0.0121

MarkerSeek score 49.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 467
variable_sites: 8
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGGTTGTTTCAGGCCCTAAA	AGCCGAAACATGGTTAGAGC	50.0	50.0	58.0	58.3	927 bp	1.0	79.0
psaC-ndhE_p2	GGGTTGTTTCAGGCCCTAAA	GCCGAAACATGGTTAGAGCT	50.0	50.0	58.0	58.3	926 bp	1.0	79.0
psaC-ndhE_p3	CGATTCCCAATCTCCAGTGG	AGCCGAAACATGGTTAGAGC	55.0	50.0	58.0	58.3	1316 bp	1.0	78.7
psaC-ndhE_p4	CGATTCCCAATCTCCAGTGG	GCCGAAACATGGTTAGAGCT	55.0	50.0	58.0	58.3	1315 bp	1.0	78.7
psaC-ndhE_p5	ACACAATCTTCGGTTCTCGG	GCCGAAACATGGTTAGAGCT	50.0	50.0	57.9	58.3	849 bp	1.0	78.3