Hotspot

ccsA-ndhD

SSC · 106754-106946 · 193 bp

Back to Aeluropus

Pi 0.0259

MarkerSeek score 68.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 193
variable_sites: 7
pi: 0.0259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCATTTGTAGCTTCGATAGGA	CTCTCGCTATCCGTTGACAG	40.9	55.0	57.6	57.9	362 bp	1.0	61.4
ccsA-ndhD_p2	TGCATTTGTAGCTTCGATAGGA	TTGACAGGGTAGAGGCTCTC	40.9	55.0	57.6	58.1	349 bp	1.0	61.2
ccsA-ndhD_p3	TGCATTTGTAGCTTCGATAGGA	GACAGGGTAGAGGCTCTCTT	40.9	55.0	57.6	57.8	347 bp	1.0	61.2
ccsA-ndhD_p4	TGCATTTGTAGCTTCGATAGGA	TCTCTCGCTATCCGTTGACA	40.9	50.0	57.6	58.3	363 bp	1.0	60.3
ccsA-ndhD_p5	TTCTGCATTTGTAGCTTCGA	CTCTCGCTATCCGTTGACAG	40.0	55.0	55.4	57.9	365 bp	1.0	59.8