Hotspot

rpl32-trnL-UAG

SSC · 105095-105627 · 533 bp

Back to Aeluropus

Pi 0.0175

MarkerSeek score 63.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 533
variable_sites: 14
pi: 0.0175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATTTTTGAGTGGCAACGAGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.6	57.9	621 bp	1.0	77.4
rpl32-trnL-UAG_p2	ATTTTTGAGTGGCAACGAGC	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.6	57.8	614 bp	1.0	77.0
rpl32-trnL-UAG_p3	ATTTTTGAGTGGCAACGAGC	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.6	58.2	635 bp	1.0	76.9
rpl32-trnL-UAG_p4	ATTTTTGAGTGGCAACGAGC	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.6	57.8	620 bp	1.0	76.9
rpl32-trnL-UAG_p5	ATTTTTGAGTGGCAACGAGC	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.6	57.8	619 bp	1.0	76.9