Hotspot

petA-psbJ

LSC · 60864-61787 · 924 bp

Back to Aeluropus

Pi 0.00652

MarkerSeek score 63.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 9
pi: 0.00652
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1064-1068 bp	1.0	66.7
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	55.0	47.6	58.8	58.1	1075-1079 bp	1.0	65.9
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1062-1066 bp	1.0	65.3
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	1120-1124 bp	1.0	64.5
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	1121-1125 bp	1.0	64.5