Hotspot

ycf4-cemA

LSC · 58612-58988 · 377 bp

Back to Aeluropus

Pi 0.0125

MarkerSeek score 54.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 7
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTAGCCTATTTCTTGCGCGT	AGGAAAGAAGGCAATGCTTTC	45.0	42.9	58.0	57.1	438-471 bp	1.0	67.3
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	AGGAAAGAAGGCAATGCTTTC	45.0	42.9	57.9	57.1	425-458 bp	1.0	66.8
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	AGGAAAGAAGGCAATGCTTTC	50.0	42.9	58.1	57.1	427-460 bp	1.0	66.5
ycf4-cemA_p4	AAAAGCTGCCGAATTAGCCT	AGGAAAGAAGGCAATGCTTTC	45.0	42.9	58.2	57.1	451-484 bp	1.0	66.0
ycf4-cemA_p5	TTAGCCTATTTCTTGCGCGT	AGGAAAGAAGGCAATGCTTT	45.0	40.0	58.0	55.8	438-471 bp	1.0	65.2