Hotspot

trnC-GCA-rpoB

LSC · 18821-19857 · 1037 bp

Back to Aeluropus

Pi 0.009

MarkerSeek score 54.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 13
pi: 0.009
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1121-1133 bp	1.0	78.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1120-1132 bp	1.0	78.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1104-1116 bp	1.0	77.5
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1105-1117 bp	1.0	77.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCCGGGTATTGTGGACATTC	50.0	50.0	58.1	57.3	1122-1134 bp	1.0	75.2