Hotspot

psbK-psbI

LSC · 7444-7841 · 398 bp

Back to Aeluropus

Pi 0.00838

MarkerSeek score 53.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 398
variable_sites: 5
pi: 0.00838
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTACTCCTCACGTCCAGGA	50.0	50.0	58.0	57.7	589-590 bp	1.0	78.1
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTACTCCTCACGTCCAGGA	50.0	50.0	57.9	57.7	540-541 bp	1.0	77.9
psbK-psbI_p3	ACCCGAAGCTTATGCCATTT	TTTACTCCTCACGTCCAGGA	45.0	50.0	57.9	57.7	619-620 bp	1.0	77.1
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	TTTACTCCTCACGTCCAGGA	45.0	50.0	58.2	57.7	549-550 bp	1.0	77.1
psbK-psbI_p5	AGCCAAATTACCCGAAGCTT	TTTACTCCTCACGTCCAGGA	45.0	50.0	57.8	57.7	628-629 bp	1.0	76.5