Hotspot

rps16-trnQ-UUG

LSC · 5577-6832 · 1256 bp

Back to Aeluropus

Pi 0.0106
MarkerSeek score 65.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1256
variable_sites
20
pi
0.0106
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1330-1339 bp 1.0 79.7
rps16-trnQ-UUG_p2 TCATCTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1341-1350 bp 1.0 77.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CGCATAAAACGAAATGGGGC 45.0 50.0 58.1 58.4 1392-1401 bp 1.0 76.7
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GCATAAAACGAAATGGGGCG 45.0 50.0 58.1 58.4 1391-1400 bp 1.0 76.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1334-1343 bp 1.0 75.0