Hotspot

atpE

Genome · 15276-15656 · 381 bp

Back to Aeginetia

Pi 0.326

MarkerSeek score 56.00

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: Genome
length_bp: 381
variable_sites: 14
pi: 0.326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.11
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	CGGCAAAAGTCATTGGTTCA	ACCTAGAATCGTAGGGGAGC	45.0	55.0	57.2	58.0	942 bp	0.5	53.7
atpE_p2	CGGCAAAAGTCATTGGTTCA	CCTAGAATCGTAGGGGAGCA	45.0	55.0	57.2	58.0	941 bp	0.5	53.6
atpE_p3	ACGGCAAAAGTCATTGGTTC	ACCTAGAATCGTAGGGGAGC	45.0	55.0	57.2	58.0	943 bp	0.5	53.6
atpE_p4	ACGGCAAAAGTCATTGGTTC	CCTAGAATCGTAGGGGAGCA	45.0	55.0	57.2	58.0	942 bp	0.5	53.6
atpE_p5	CGGCAAAAGTCATTGGTTCA	CTGCTCCTGCGACTACATTT	45.0	50.0	57.2	58.0	1062 bp	0.5	53.5