Hotspot

rrn16-rrn23

Genome · 43950-45351 · 1402 bp

Back to Aeginetia

Pi 0.0572

MarkerSeek score 81.60

Primer available yes

Label rrn16-rrn23

Feature Metadata

label_name: rrn16-rrn23
feature_type: igs
region: Genome
length_bp: 1402
variable_sites: 77
pi: 0.0572
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rrn23_p1	CAAGGTAGCCGTACTGGAAG	TCGATTACTACGCCCTTCCT	55.0	50.0	57.7	57.9	1466-1495 bp	1.0	84.4
rrn16-rrn23_p2	CAAGGTAGCCGTACTGGAAG	GTCTCTGGGTGCCTAGGTAT	55.0	55.0	57.7	57.9	1445-1474 bp	1.0	84.3
rrn16-rrn23_p3	AGTGACTGGAGTGAAGTCGT	TCGATTACTACGCCCTTCCT	50.0	50.0	58.3	57.9	1488-1517 bp	1.0	84.2
rrn16-rrn23_p4	AGTGACTGGAGTGAAGTCGT	GTCTCTGGGTGCCTAGGTAT	50.0	55.0	58.3	57.9	1467-1496 bp	1.0	84.1
rrn16-rrn23_p5	AAGTCGTAACAAGGTAGCCG	TCGATTACTACGCCCTTCCT	50.0	50.0	57.6	57.9	1475-1504 bp	1.0	83.8