Hotspot

rps11-rpl36

Genome · 23795-23878 · 84 bp

Back to Aeginetia

Pi 0.177

MarkerSeek score 79.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: Genome
length_bp: 84
variable_sites: 14
pi: 0.177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCAGTTTTTCGTGAATTAGTTCT	AGGCATAAACAAAGACAGGGA	30.4	42.9	54.5	57.2	149-153 bp	1.0	50.1
rps11-rpl36_p2	TCAGTTTTTCGTGAATTAGTTCTT	AGGCATAAACAAAGACAGGGA	29.2	42.9	55.3	57.2	149-153 bp	1.0	50.1
rps11-rpl36_p3	TCAGTTTTTCGTGAATTAGTTCT	AAGGCATAAACAAAGACAGGGA	30.4	40.9	54.5	57.9	150-154 bp	1.0	50.0
rps11-rpl36_p4	TCAGTTTTTCGTGAATTAGTTCT	CAAGGCATAAACAAAGACAGGG	30.4	45.5	54.5	57.8	151-155 bp	1.0	49.9
rps11-rpl36_p5	TCAGTTTTTCGTGAATTAGTTCT	CCAAGGCATAAACAAAGACAGG	30.4	45.5	54.5	57.8	152-156 bp	1.0	49.9