Hotspot

rps12-clpP

Genome · 21364-21482 · 119 bp

Back to Aeginetia

Pi 0.152

MarkerSeek score 78.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 119
variable_sites: 16
pi: 0.152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ATAAGTTGTTTAATAGTTGGCAT	TGGAATTGTTGATCTTGTAGCA	26.1	36.4	52.4	56.0	173-194 bp	1.0	51.0
rps12-clpP_p2	AATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCA	26.1	36.4	52.6	56.0	174-195 bp	1.0	50.9
rps12-clpP_p3	AATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCAA	26.1	34.8	52.6	56.7	174-195 bp	1.0	50.9
rps12-clpP_p4	AATAAGTTGTTTAATAGTTGGCAT	TGGAATTGTTGATCTTGTAGCA	25.0	36.4	53.2	56.0	174-195 bp	1.0	50.9
rps12-clpP_p5	TCTAATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCA	26.9	36.4	55.2	56.0	177-198 bp	1.0	50.8