Hotspot

atpF-atpH

Genome · 6434-6746 · 313 bp

Back to Aeginetia

Pi 0.462

MarkerSeek score 69.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 313
variable_sites: 92
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AACTCGGCGGTAAAACAACT	GAGACAACTCGAGGCTGAAG	45.0	55.0	58.0	58.0	824 bp	0.5	59.6
atpF-atpH_p2	AACTCGGCGGTAAAACAACT	TGACCCTGACCTTGCAGATA	45.0	50.0	58.0	58.0	1217 bp	0.5	59.3
atpF-atpH_p3	AACTCGGCGGTAAAACAACT	TGTAGGGCTTGCTTCTTTGG	45.0	50.0	58.0	58.1	887 bp	0.5	58.9
atpF-atpH_p4	AACTCGGCGGTAAAACAACT	ATTTCTGCTGCTTCTGTGCT	45.0	45.0	58.0	58.1	922 bp	0.5	58.9
atpF-atpH_p5	AACTCGGCGGTAAAACAACT	CAACTCGAGGCTGAAGGAAA	45.0	50.0	58.0	57.8	820 bp	0.5	58.3