Hotspot

rps16-trnQ-UUG

Genome · 2831-3869 · 1039 bp

Back to Aeginetia

Pi 0.0918

MarkerSeek score 85.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 1039
variable_sites: 87
pi: 0.0918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CTCTTTTCCTTCTCGTCGGG	CGTAGGTTCGAATCCTTCCG	55.0	55.0	58.0	58.2	1126-1208 bp	1.0	88.6
rps16-trnQ-UUG_p2	CTCTTTTCCTTCTCGTCGGG	TGGCTAAGTGGTAAGGCAAC	55.0	50.0	58.0	57.8	1165-1247 bp	1.0	88.2
rps16-trnQ-UUG_p3	CTCTTTTCCTTCTCGTCGGG	CTAAGTGGTAAGGCAACGGG	55.0	55.0	58.0	58.3	1162-1244 bp	1.0	87.6
rps16-trnQ-UUG_p4	AGCTCTTTTCCTTCTCGTCG	CGTAGGTTCGAATCCTTCCG	50.0	55.0	57.6	58.2	1128-1210 bp	1.0	85.8
rps16-trnQ-UUG_p5	TCTTTTCCTTCTCGTCGGGA	CGTAGGTTCGAATCCTTCCG	50.0	55.0	58.4	58.2	1125-1207 bp	1.0	85.7