Hotspot

trnH-GUG-matK

Genome · 75-609 · 535 bp

Back to Aeginetia

Pi 0.13

MarkerSeek score 81.70

Primer available yes

Label trnH-GUG-matK

Feature Metadata

label_name: trnH-GUG-matK
feature_type: igs
region: Genome
length_bp: 535
variable_sites: 64
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-matK_p1	TTGCCTTCATCCATTTGGCT	TCTGGCTTCAAAAGGAACGT	45.0	45.0	58.1	57.7	1236-1388 bp	1.0	85.9
trnH-GUG-matK_p2	TTGCCTTCATCCATTTGGCT	TGGCTTCAAAAGGAACGTCT	45.0	45.0	58.1	57.7	1234-1386 bp	1.0	85.9
trnH-GUG-matK_p3	TTGCCTTCATCCATTTGGCT	TGGGGCATTGACTAAAGCAA	45.0	45.0	58.1	57.7	972 bp	0.5	57.1
trnH-GUG-matK_p4	TTGCCTTCATCCATTTGGCT	TTGGGGCATTGACTAAAGCA	45.0	45.0	58.1	57.7	973 bp	0.5	57.1
trnH-GUG-matK_p5	TTGCCTTCATCCATTTGGCT	AGCGAACCTTTGGTTGGTTA	45.0	45.0	58.1	57.6	1299 bp	0.5	56.7