Hotspot

ccsA-ndhD

SSC · 107767-107932 · 166 bp

Back to Aegilops

Pi 0.0406

MarkerSeek score 64.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 166
variable_sites: 14
pi: 0.0406
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCTGCACTTATAGCTTCG	TTGACAGGGTAGAGGCTCTC	43.5	55.0	58.0	58.1	324-329 bp	1.0	59.7
ccsA-ndhD_p2	CGAATTCTGCACTTATAGCTTCG	GACAGGGTAGAGGCTCTCTT	43.5	55.0	58.0	57.8	322-327 bp	1.0	59.7
ccsA-ndhD_p3	CGAATTCTGCACTTATAGCTTCG	CCCTATCCGTTGACAGGGTA	43.5	55.0	58.0	58.2	333-338 bp	1.0	59.0
ccsA-ndhD_p4	CGAATTCTGCACTTATAGCTTCG	TCTCCCTATCCGTTGACAGG	43.5	55.0	58.0	58.2	336-341 bp	1.0	58.9
ccsA-ndhD_p5	CGAATTCTGCACTTATAGCTTCG	GTTGACAGGGTAGAGGCTCT	43.5	55.0	58.0	58.4	325-330 bp	1.0	57.4