Hotspot

rpl32-LK95_t054

SSC · 105933-106646 · 714 bp

Back to Aegilops

Pi 0.00715

MarkerSeek score 38.40

Primer available yes

Label rpl32-LK95_t054

Feature Metadata

label_name: rpl32-LK95_t054
feature_type: igs
region: SSC
length_bp: 714
variable_sites: 16
pi: 0.00715
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-LK95_t054_p1	GAGCGGTAATGAGCATCCAA	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	845-871 bp	1.0	82.0
rpl32-LK95_t054_p2	TTTTCGAGCGGTAATGAGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.9	58.0	850-876 bp	1.0	81.7
rpl32-LK95_t054_p3	GAGCGGTAATGAGCATCCAA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	848-874 bp	1.0	81.6
rpl32-LK95_t054_p4	GAGCGGTAATGAGCATCCAA	GATCCGCACAAAAGAGTTGC	50.0	50.0	58.0	58.0	1391-1417 bp	1.0	80.9
rpl32-LK95_t054_p5	TTTTCGAGCGGTAATGAGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.9	58.0	1396-1422 bp	1.0	80.6