Hotspot

psbE-petL

LSC · 62808-63971 · 1164 bp

Back to Aegilops

Pi 0.006

MarkerSeek score 55.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 19
pi: 0.006
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	45.0	50.0	58.0	58.3	922-1375 bp	1.0	83.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	50.0	50.0	57.9	58.3	911-1364 bp	1.0	82.7
psbE-petL_p3	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	50.0	50.0	58.2	58.3	913-1366 bp	1.0	81.3
psbE-petL_p4	TGACAAATAACCAACCCGCA	GGCGTATCTTGTTCAAGCCA	45.0	50.0	58.0	58.6	921-1374 bp	1.0	80.7
psbE-petL_p5	CAACCCGCAATGAATAGGGA	GGCGTATCTTGTTCAAGCCA	50.0	50.0	57.9	58.6	910-1363 bp	1.0	80.4