Hotspot

rbcL-rpl23

LSC · 56181-56491 · 311 bp

Back to Aegilops

Pi 0.00948
MarkerSeek score 51.30
Primer available yes
Label rbcL-rpl23

Feature Metadata

label_name
rbcL-rpl23
feature_type
igs
region
LSC
length_bp
311
variable_sites
8
pi
0.00948
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-rpl23_p1 CGCAGCTTGTGAAGTATGGA AGAGTTCGACCCAATGCTTT 50.0 45.0 58.3 57.4 485-496 bp 0.769231 67.6
rbcL-rpl23_p2 AGTTCGAGCCGGTAGATACT AGAGTTCGACCCAATGCTTT 50.0 45.0 57.7 57.4 450-461 bp 0.769231 67.3
rbcL-rpl23_p3 GGCGATCAAATTCGAGTTCG AGAGTTCGACCCAATGCTTT 50.0 45.0 57.6 57.4 464-475 bp 0.769231 67.1
rbcL-rpl23_p4 TGGAAGGCGATCAAATTCGA AGAGTTCGACCCAATGCTTT 45.0 45.0 57.6 57.4 469-480 bp 0.769231 66.8
rbcL-rpl23_p5 AGCTTGCAAATGGAGTCCTG AGAGTTCGACCCAATGCTTT 50.0 45.0 58.5 57.4 512-523 bp 0.769231 66.0