Hotspot

LK95_t030-LK95_t032

LSC · 46141-47768 · 1628 bp

Back to Aegilops

Pi 0.00131

MarkerSeek score 53.60

Primer available yes

Label LK95_t030-LK95_t032

Feature Metadata

label_name: LK95_t030-LK95_t032
feature_type: igs
region: LSC
length_bp: 1628
variable_sites: 9
pi: 0.00131
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
LK95_t030-LK95_t032_p1	ATTTGAACCGATGACCCTCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.0	58.1	1747-1788 bp	1.0	80.5
LK95_t030-LK95_t032_p2	CGATGACCCTCGCATTACAA	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	1739-1780 bp	1.0	80.2
LK95_t030-LK95_t032_p3	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1717-1758 bp	1.0	79.6
LK95_t030-LK95_t032_p4	ATTTGAACCGATGACCCTCG	CTGCCAGGAACCAGATTTGA	50.0	50.0	58.0	57.8	1762-1803 bp	1.0	79.3
LK95_t030-LK95_t032_p5	ATTTGAACCGATGACCCTCG	TCTGCCAGGAACCAGATTTG	50.0	50.0	58.0	57.8	1763-1804 bp	1.0	79.3