Hotspot

psbM-petN

LSC · 16739-17370 · 632 bp

Back to Aegilops

Pi 0.00235
MarkerSeek score 49.80
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
632
variable_sites
6
pi
0.00235
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTCATTCT AGTATGGGGAAGGAGTGGAC 42.9 55.0 57.6 58.1 731-753 bp 1.0 69.6
psbM-petN_p2 TGCTACTGCACTGTTCATTCT GTATGGGGAAGGAGTGGACT 42.9 55.0 57.6 58.1 730-752 bp 1.0 69.6
psbM-petN_p3 TGCTACTGCACTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 42.9 50.0 57.6 58.2 789-811 bp 1.0 69.2
psbM-petN_p4 TGCTACTGCACTGTTCATTCT TATGGGGAAGGAGTGGACTC 42.9 55.0 57.6 57.8 729-751 bp 1.0 69.2
psbM-petN_p5 TGCTACTGCACTGTTCATTCT GGGGAAGGAGTGGACTCTAG 42.9 60.0 57.6 58.2 726-748 bp 1.0 68.9