Hotspot

ycf1

SSC · 121033-126114 · 5082 bp

Back to Adromischus

Pi 0.0104

MarkerSeek score 45.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5082
variable_sites: 103
pi: 0.0104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGTATACCACTTTTTGTTAAACCT	AAAAATCAGATAATTCATGAATCG	36.0	25.0	57.8	52.3	202-205 bp	1.0	43.2
ycf1_p2	CGGTATACCACTTTTTGTTAAACC	AAAAATCAGATAATTCATGAATCG	37.5	25.0	56.5	52.3	202-205 bp	1.0	43.2
ycf1_p3	ACGGTATACCACTTTTTGTTAAACC	AAAAATCAGATAATTCATGAATCG	36.0	25.0	58.0	52.3	203-206 bp	1.0	43.1
ycf1_p4	ACGGTATACCACTTTTTGTT	AAAAATCAGATAATTCATGAATCG	35.0	25.0	52.7	52.3	203-206 bp	1.0	43.1
ycf1_p5	TACGGTATACCACTTTTTGTTAAACC	AAAAATCAGATAATTCATGAATCG	34.6	25.0	57.9	52.3	204-207 bp	1.0	43.1