Hotspot

rps3

LSC · 81405-82061 · 657 bp

Back to Adromischus

Pi 0.00228

MarkerSeek score 38.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 3
pi: 0.00228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GGGAACAGAAATGAAACTGAAGG	ATGTATAGTAGTGAGGGAGC	43.5	45.0	57.9	53.0	704 bp	1.0	40.3
rps3_p2	GGGAACAGAAATGAAACTGAAGG	TGATATGTATAGTAGTGAGGGAGC	43.5	41.7	57.9	56.7	708 bp	1.0	40.3
rps3_p3	GGGAACAGAAATGAAACTGAAGG	ACACAGATATGTCGTATCATGA	43.5	36.4	57.9	54.6	727 bp	1.0	40.3
rps3_p4	GAGGGAACAGAAATGAAACTGA	ATGTATAGTAGTGAGGGAGC	40.9	45.0	56.3	53.0	706 bp	1.0	40.3
rps3_p5	GGAACAGAAATGAAACTGAAGGT	ATGTATAGTAGTGAGGGAGC	39.1	45.0	57.2	53.0	703 bp	1.0	40.3