Hotspot

ccsA-ndhD

SSC · 112770-113014 · 245 bp

Back to Adromischus

Pi 0.0354

MarkerSeek score 64.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 14
pi: 0.0354
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGTATTCTTTCCGGAGCAG	AATGGGCGGGATAGCTATCT	55.0	50.0	58.0	58.0	917-933 bp	1.0	81.4
ccsA-ndhD_p2	GGGTATTCTTTCCGGAGCAG	ATGGGCGGGATAGCTATCTT	55.0	50.0	58.0	58.0	916-932 bp	1.0	81.4
ccsA-ndhD_p3	GGGTATTCTTTCCGGAGCAG	ACTTGTGTAATGGCACTGGG	55.0	50.0	58.0	58.1	751-767 bp	1.0	81.3
ccsA-ndhD_p4	GGATTTGCCACTTCGAGTCT	AATGGGCGGGATAGCTATCT	50.0	50.0	57.9	58.0	1291-1307 bp	1.0	80.6
ccsA-ndhD_p5	GGATTTGCCACTTCGAGTCT	ATGGGCGGGATAGCTATCTT	50.0	50.0	57.9	58.0	1290-1306 bp	1.0	80.6