Hotspot

rps3-rpl22

LSC · 82062-82192 · 131 bp

Back to Adromischus

Pi 0.0878
MarkerSeek score 77.80
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
131
variable_sites
17
pi
0.0878
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 CGACACCGGAAGCTATTCTC TCCCGGGCATCTACCATTAT 55.0 50.0 58.2 58.0 890-946 bp 1.0 82.9
rps3-rpl22_p2 CTCGACACCGGAAGCTATTC TCCCGGGCATCTACCATTAT 55.0 50.0 58.2 58.0 892-948 bp 1.0 82.9
rps3-rpl22_p3 CTCGGATCCATTCAACACGT TCCCGGGCATCTACCATTAT 50.0 50.0 58.0 58.0 1259-1315 bp 1.0 82.8
rps3-rpl22_p4 CTGCCTTCTCGGATCCATTC TCCCGGGCATCTACCATTAT 55.0 50.0 58.1 58.0 1266-1322 bp 1.0 81.9
rps3-rpl22_p5 CTCGGATCCATTCAACACGT ACAGATCGTATGGTCGGACA 50.0 50.0 58.0 58.2 1175-1231 bp 1.0 81.3