Hotspot

rps11-rpl36

LSC · 78078-78195 · 118 bp

Back to Adromischus

Pi 0.0268

MarkerSeek score 63.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 6
pi: 0.0268
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GGAATTCTGCGTCCACTCTT	CCAACCCGAGACATAAGCAA	50.0	50.0	57.9	57.9	227-252 bp	1.0	80.4
rps11-rpl36_p2	CGGTTTTGCGTGAACCAATT	CCAACCCGAGACATAAGCAA	45.0	50.0	58.2	57.9	190-215 bp	1.0	80.4
rps11-rpl36_p3	GGAATTCTGCGTCCACTCTT	AAATTGTCGACTGATCCGCA	50.0	45.0	57.9	57.9	274-299 bp	1.0	80.2
rps11-rpl36_p4	CGGTTTTGCGTGAACCAATT	AAATTGTCGACTGATCCGCA	45.0	45.0	58.2	57.9	237-262 bp	1.0	80.0
rps11-rpl36_p5	CTCCCTTTGGAATTCTGCGT	AAATTGTCGACTGATCCGCA	50.0	45.0	58.2	57.9	282-307 bp	1.0	79.5