Hotspot

petG-trnW-CCA

LSC · 65101-65219 · 119 bp

Back to Adromischus

Pi 0.0224

MarkerSeek score 64.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 5
pi: 0.0224
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	AACGTGGTGATCAGTTGGAC	GGTTCAAGTCCTACAGAGCG	50.0	55.0	58.1	58.0	167 bp	1.0	81.4
petG-trnW-CCA_p2	CGTGGTGATCAGTTGGACTT	GGTTCAAGTCCTACAGAGCG	50.0	55.0	57.8	58.0	165 bp	1.0	81.0
petG-trnW-CCA_p3	AACGTGGTGATCAGTTGGAC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	198 bp	1.0	80.8
petG-trnW-CCA_p4	AACGTGGTGATCAGTTGGAC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	220 bp	1.0	80.7
petG-trnW-CCA_p5	CGTGGTGATCAGTTGGACTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	218 bp	1.0	80.3