Hotspot

psbE-petL

LSC · 63554-64716 · 1163 bp

Back to Adromischus

Pi 0.00849

MarkerSeek score 66.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 19
pi: 0.00849
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.9	58.0	1345-1353 bp	1.0	81.6
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1268-1276 bp	1.0	80.9
psbE-petL_p3	ACGAATAACCAACCCGCAAT	CGTATCTTGCTCAGCCCAAT	45.0	50.0	58.2	58.0	1354-1362 bp	1.0	80.7
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	CGTATCTTGCTCAGCCCAAT	55.0	50.0	58.2	58.0	1263-1271 bp	1.0	80.6
psbE-petL_p5	CGAATAACCAACCCGCAATG	CGTATCTTGCTCAGCCCAAT	50.0	50.0	57.8	58.0	1353-1361 bp	1.0	80.5