Hotspot

petA-psbJ

LSC · 61910-62776 · 867 bp

Back to Adromischus

Pi 0.0258

MarkerSeek score 77.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 44
pi: 0.0258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CAGGATTGGGTTCATCCTTGT	50.0	47.6	57.8	57.9	984-1018 bp	1.0	74.6
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	CAGGATTGGGTTCATCCTTGT	50.0	47.6	58.2	57.9	999-1033 bp	1.0	74.3
petA-psbJ_p3	TCCATTACGTGTTCAAGGCC	CAGGATTGGGTTCATCCTTGT	50.0	47.6	58.2	57.9	1000-1034 bp	1.0	74.3
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	1036-1070 bp	1.0	72.9
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1035-1069 bp	1.0	72.9