Hotspot

psaI-cemA

LSC · 58633-60032 · 1400 bp

Back to Adromischus

Pi 0.00631

MarkerSeek score 64.50

Primer available yes

Label psaI-cemA

Feature Metadata

label_name: psaI-cemA
feature_type: igs
region: LSC
length_bp: 1400
variable_sites: 17
pi: 0.00631
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-cemA_p1	GCAATGGCAATGGCTTCTTT	AAGAGAAATCAACCAGGGCA	45.0	45.0	57.9	57.0	1516-1545 bp	1.0	73.1
psaI-cemA_p2	GCAATGGCAATGGCTTCTTT	AGAGAAATCAACCAGGGCAA	45.0	45.0	57.9	57.0	1515-1544 bp	1.0	73.1
psaI-cemA_p3	GTTTTTCCGGCAATGGCAAT	AAGAGAAATCAACCAGGGCA	45.0	45.0	58.2	57.0	1525-1554 bp	1.0	72.4
psaI-cemA_p4	GTTTTTCCGGCAATGGCAAT	AGAGAAATCAACCAGGGCAA	45.0	45.0	58.2	57.0	1524-1553 bp	1.0	72.4
psaI-cemA_p5	TTTTTCCGGCAATGGCAATG	AGAGAAATCAACCAGGGCAA	45.0	45.0	58.2	57.0	1523-1552 bp	1.0	72.4