Hotspot

rbcL-accD

LSC · 55823-56417 · 595 bp

Back to Adromischus

Pi 0.0101

MarkerSeek score 65.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 12
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCGAGGCTTGTAAATGGAGT	TGAACCGCCGTTTTTCCATA	50.0	45.0	58.3	58.1	784-791 bp	1.0	78.3
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	TGAACCGCCGTTTTTCCATA	55.0	45.0	57.7	58.1	768-775 bp	1.0	78.1
rbcL-accD_p3	GCGAGGCTTGTAAATGGAGT	AATTGAACCGCCGTTTTTCC	50.0	45.0	58.3	57.9	787-794 bp	1.0	78.0
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	AATTGAACCGCCGTTTTTCC	55.0	45.0	57.7	57.9	771-778 bp	1.0	77.8
rbcL-accD_p5	GCGAGGCTTGTAAATGGAGT	AACCGCCGTTTTTCCATAGA	50.0	45.0	58.3	57.8	782-789 bp	1.0	77.6