Hotspot

psbZ-trnG-GCC

LSC · 36165-36768 · 604 bp

Back to Adromischus

Pi 0.0254

MarkerSeek score 66.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 30
pi: 0.0254
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	691-751 bp	1.0	73.4
psbZ-trnG-GCC_p2	AGTCTTTCTGGTGGGTATCCT	TACCATTCGACCATATCCGC	47.6	50.0	57.8	57.3	664-724 bp	1.0	69.0
psbZ-trnG-GCC_p3	AGTCTTTCTGGTGGGTATCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	691-751 bp	1.0	68.8
psbZ-trnG-GCC_p4	GTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	690-750 bp	1.0	68.8
psbZ-trnG-GCC_p5	TGGATTAGTCTTTCTGGTGGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	697-757 bp	1.0	67.0