Hotspot

rpoB-trnC-GCA

LSC · 26915-28048 · 1134 bp

Back to Adromischus

Pi 0.0159
MarkerSeek score 63.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1134
variable_sites
36
pi
0.0159
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCTCATTTCCATCCCG CCGGATTTGAACTGGGGAAA 50.0 50.0 57.3 58.1 1204-1222 bp 1.0 78.2
rpoB-trnC-GCA_p2 ATTCCCTCATTTCCATCCCG GAGTCTTGGGTTGATCAGGC 50.0 55.0 57.3 58.3 1229-1247 bp 1.0 76.8
rpoB-trnC-GCA_p3 ATTCCCTCATTTCCATCCCG AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.3 58.4 1186-1204 bp 1.0 76.1
rpoB-trnC-GCA_p4 GTGGACATTCCCTCATTTCCA CCGGATTTGAACTGGGGAAA 47.6 50.0 57.9 58.1 1210-1228 bp 1.0 74.9
rpoB-trnC-GCA_p5 TGTGGACATTCCCTCATTTCC CCGGATTTGAACTGGGGAAA 47.6 50.0 57.9 58.1 1211-1229 bp 1.0 74.9