Hotspot

trnS-GCU-trnG-GCC

LSC · 8824-9346 · 523 bp

Back to Adromischus

Pi 0.0147

MarkerSeek score 65.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 14
pi: 0.0147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	608-711 bp	1.0	60.0
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	607-710 bp	1.0	60.0
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.1	57.8	626-729 bp	1.0	59.4
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.2	57.8	662-765 bp	1.0	58.7
trnS-GCU-trnG-GCC_p5	GAATCGCTCGTACAACGGAT	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.2	57.8	631-734 bp	1.0	58.7