Hotspot

trnK-UUU-rps16

LSC · 4191-4911 · 721 bp

Back to Adromischus

Pi 0.00902
MarkerSeek score 63.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
721
variable_sites
13
pi
0.00902
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GTGCTAAACCGACAGGAACT 50.0 50.0 58.0 57.8 895-898 bp 1.0 80.2
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GTGCTAAACCGACAGGAACT 50.0 50.0 58.0 57.8 896-899 bp 1.0 80.2
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GTGCTAAACCGACAGGAACT 50.0 50.0 58.0 57.8 900-903 bp 1.0 80.2
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GTGCTAAACCGACAGGAACT 50.0 50.0 58.0 57.8 898-901 bp 1.0 80.2
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC GTGCTAAACCGACAGGAACT 55.0 50.0 57.9 57.8 907-910 bp 1.0 79.9