Hotspot

ycf1

SSC · 126596-131857 · 5262 bp

Pi 0.0112

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5262
variable_sites: 118
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCCACTCTTGCCAATCCTC	ACAACCTGAGCAACGAACTT	55.0	45.0	58.0	58.0	1162-1174 bp	1.0	82.2
ycf1_p2	CTCCACTCTTGCCAATCCTC	AACAACCTGAGCAACGAACT	55.0	45.0	58.0	58.0	1163-1175 bp	1.0	82.1
ycf1_p3	AAAGCGGTGAATGCACATTG	TTGATTGACGGTTTGGCTGA	45.0	45.0	57.9	58.0	280-286 bp	0.8	76.1
ycf1_p4	CTCCACTCTTGCCAATCCTC	TTGATTGACGGTTTGGCTGA	55.0	45.0	58.0	58.0	632-638 bp	0.8	74.5
ycf1_p5	CTCTTGCCAATCCTCGTACC	TTGATTGACGGTTTGGCTGA	55.0	45.0	58.1	58.0	627-633 bp	0.8	74.4