Hotspot

ccsA-ndhD

SSC · 117839-118099 · 261 bp

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Pi 0.023
MarkerSeek score 60.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
261
variable_sites
11
pi
0.023
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCAATTGTGGCTTCTATGGG TCCGGATTTCATTCTCTCACT 50.0 42.9 57.1 56.5 440-497 bp 1.0 55.0
ccsA-ndhD_p2 GCAATTGTGGCTTCTATGGG ATCCGGATTTCATTCTCTCACT 50.0 40.9 57.1 57.0 441-498 bp 1.0 51.1
ccsA-ndhD_p3 CTGCAATTGTGGCTTCTATGG TCCGGATTTCATTCTCTCACT 47.6 42.9 57.6 56.5 442-499 bp 1.0 50.6
ccsA-ndhD_p4 GCAATTGTGGCTTCTATGGG TCCGGATTTCATTCTCTCAC 50.0 45.0 57.1 54.9 440-497 bp 1.0 50.4
ccsA-ndhD_p5 GCAATTGTGGCTTCTATGGG CCGGATTTCATTCTCTCACT 50.0 45.0 57.1 54.9 439-496 bp 1.0 50.3