Hotspot

ndhF-trnL-UAG

SSC · 114812-116663 · 1852 bp

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Pi 0.0137
MarkerSeek score 60.20
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1852
variable_sites
49
pi
0.0137
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 ACGAAAGGTATGATCCACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1922-1943 bp 1.0 82.1
ndhF-trnL-UAG_p2 CGAAAGGTATGATCCACGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1921-1942 bp 1.0 82.1
ndhF-trnL-UAG_p3 ACGAAAGGTATGATCCACGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1923-1944 bp 1.0 82.1
ndhF-trnL-UAG_p4 CGAAAGGTATGATCCACGCA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1922-1943 bp 1.0 82.1
ndhF-trnL-UAG_p5 ACGAAAGGTATGATCCACGC TTGATAAGATGCCGCCACTC 50.0 50.0 58.1 58.0 1982-2003 bp 1.0 81.7