Hotspot

atpH-atpF

LSC · 39389-39844 · 456 bp

Pi 0.0202

MarkerSeek score 62.00

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 20
pi: 0.0202
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCGCTTTTATTTGCAAATCCC	CAGTGACCCAAGGAAACGAA	42.9	50.0	57.3	58.0	526-541 bp	1.0	71.7
atpH-atpF_p2	GGCATTAGCGCTTTTATTTGC	CAGTGACCCAAGGAAACGAA	42.9	50.0	57.2	58.0	533-548 bp	1.0	70.2
atpH-atpF_p3	GCGCTTTTATTTGCAAATCCC	TTAAATCCGAAACTCCCGGC	42.9	50.0	57.3	58.3	553-568 bp	1.0	69.7
atpH-atpF_p4	GGCATTAGCGCTTTTATTTGC	TTAAATCCGAAACTCCCGGC	42.9	50.0	57.2	58.3	560-575 bp	1.0	68.1
atpH-atpF_p5	GCGCTTTTATTTGCAAATCCC	AGTGACCCAAGGAAACGAAA	42.9	45.0	57.3	57.3	525-540 bp	1.0	66.3