Hotspot

trnC-GCA-rpoB

LSC · 24695-25945 · 1251 bp

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Pi 0.0062
MarkerSeek score 60.90
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1251
variable_sites
16
pi
0.0062
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 CCGGATTTGAACTGGGGAAA ATTCCCTCATTTCCATCCCG 50.0 50.0 58.1 57.3 1333-1370 bp 1.0 77.7
trnC-GCA-rpoB_p2 CAAAGGGGTTTCGCGTTTTT ATTCCCTCATTTCCATCCCG 45.0 50.0 58.1 57.3 1371-1408 bp 1.0 77.6
trnC-GCA-rpoB_p3 CAAAGGGGTTTCGCGTTTTT GTTCTTCCGTCAATCCCTGA 45.0 50.0 58.1 57.2 1448-1485 bp 1.0 76.7
trnC-GCA-rpoB_p4 CCGGATTTGAACTGGGGAAA GTTCTTCCGTCAATCCCTGA 50.0 50.0 58.1 57.2 1410-1447 bp 1.0 76.7
trnC-GCA-rpoB_p5 CAAAGGGGTTTCGCGTTTTT AGTTCTTCCGTCAATCCCTG 45.0 50.0 58.1 57.2 1449-1486 bp 1.0 76.6