Hotspot

psbM-petN

LSC · 22537-23684 · 1148 bp

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Pi 0.0114
MarkerSeek score 64.20
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
1148
variable_sites
27
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGGAAGTCAATATTCTCGCA TCTTGCTTGGGCTGCTTTAA 40.0 45.0 54.5 58.0 1319-1370 bp 1.0 53.9
psbM-petN_p2 TGGAAGTCAATATTCTCGCA AGTAAGTCTTGCTTGGGCTG 40.0 50.0 54.5 57.8 1325-1376 bp 1.0 52.5
psbM-petN_p3 TGCTACTGCACTGTTTATTCT TCTTGCTTGGGCTGCTTTAA 38.1 45.0 55.2 58.0 1294-1345 bp 1.0 52.1
psbM-petN_p4 TGGAAGTCAATATTCTCGCA ATGGGGAAGAAGTGGACTCT 40.0 50.0 54.5 57.6 1265-1316 bp 1.0 51.3
psbM-petN_p5 TGGAAGTCAATATTCTCGCAT TCTTGCTTGGGCTGCTTTAA 38.1 45.0 55.1 58.0 1319-1370 bp 1.0 50.9